| TRT |  | BAB Mikroskop ve Bs200ProP İle Yapılan Bazı Yayınlar | Yayın : Detection of irradiated quail meat by using DNA comet assay and evaluation of comets by image analysis
by: Yakup Erel, Nizamettin Yazici, Sumer Özvatan, Demet Ercin, Nurcan Cetinkaya
Radiation Physics and Chemistry (02 June 2009)
Abstract
A simple technique of microgel electrophoresis of single cells (DNA Comet Assay) was used to detect DNA comets in irradiated quail meat samples. Obtained DNA comets were evaluated by both photomicrographic and image analysis.Quail meat samples were exposed to radiation doses of 0.52, 1.05, 1.45, 2.00, 2.92 and 4.00 kGy in gamma cell (Gammacell 60 Co, dose rate 1.31 kGy/h) covering the permissible limits for enzymatic decay and stored at 2 o C. The cells isolated from muscle (chest, thorax) in cold PBS were analysed using the DNA comet assay on 1, 2, 3, 4, 7, 8 and 11 day post irradiation. The cells were lysed between 2, 5 and 9 min in 2.5% SDS and electrophroesis was carried out at a voltage of 2 V/cm for 2 min. After propidium iodide staining, the slides were evaluated through a fluorescent microscope. In all irradiated samples, fragmented DNA stretched towards the anode and damaged cells appeared as a comet. All measurement data were analyzed using BS 200 ProP with software image analysis (BS 200 ProP, BAB Imaging System, Ankara, Turkey). The density of DNA in the tails increased with increasing radiation dose. However, in non-irradiated samples, the large molecules of DNA remained relatively intact and there was only minor or no migration of DNA; the cells were round or had very short tails only. The values of tail DNA %, tail length and tail moment were significantly different and identical between 0.9 and 4.0 kGy dose exposure, and also among storage times on day 1, 4 and 8. In conclusion, DNA Comet Assay EN 13784 standard method may be used for not only screening method for detection of irradiated quail meat depending on storage time and condition but also for the quantification of applied dose if it is combined with image analysis.Image analysis may provide a powerful tool for the evaluation of head and tail of comet intensity related with applied doses.
| KTÜ |  | Genotoxicity evaluation of HMG CoA reductase inhibitor rosuvastatin | RESEARCH ARTICLE
Yayın : Genotoxicity evaluation of HMG CoA reductase inhibitor rosuvastatin
Ahmet Ali Berber1, Mustafa C¸ elik2, and Hu¨ seyin Aksoy1
1Department of Biology, Faculty of Art and Sciences, Sakarya University, Sakarya, Turkey an
Genotoxicity evaluation of HMG CoA reductase inhibitor rosuvastatin
Ahmet Ali Berber1, Mustafa C¸ elik2, and Hu¨ seyin Aksoy1
1Department of Biology, Faculty of Art and Sciences, Sakarya University, Sakarya, Turkey and 2Department of Medical Biology, Faculty of Medicine,
Kahramanmaras Sutcu Imam University, Kahramanmaras, Turkey
In the comet assay,
slides were examined using a fluorescent microscope (BAB research microscope; BAB Imaging System, Ankara, Turkey).
Slides were prepared for each concentration of rosuvastatin. The comet parameters used for the evaluation are tail length, tail DNA% and tail moment. For this reason, 100 comets on each slide were analyzed with a specialized image analyses system (BS 200 ProP; BAB Imaging System) to determine these parameters.
| SİGARA DUMANINA MARUZ KALAN PASİF İÇİCİ DURUMUNDAKİ ÇOCUKLARDA DNA ... | SİGARA DUMANINA MARUZ KALAN
Mikroskopta 'Comet Görüntü işleme ve Analiz Programı Sistemi' (BAB Bs 200 Pro) programı kullanılarak değerlendirme yapılmıştır. ...
| İLAÇ UYGULAMA PERFORMANSININ DEĞERLENDİRİLMESİNDE KALİTATİF VE KANTİTATİF ANALİZ YÖNTEMLERİNİN İNCELENMESİ | Üretim
Firmamızın ve ülke ekonomisinin sağlıklı şekilde yaşaması ve gelişmesi yeterli düzeyde üretime bağlıdır.
Üretim fiziksel bir malın, araç, gereç, insan gücü ve malzemeler ile montajı veya yapımı olarak da tanımlanabilir (mühendislik tanım)
Yönetim, üretim amacı ile biraraya getirilen faktörlerin yönetilmesi ( planlanması, koordinasyonu ve kontrolü) fonksiyonudur.
Üretim Yönetimi
Mal ve hizmetlerin üretimiyle ilgili faaliyetlerin yönetimi.
Firmamızın elinde bulunan malzeme, makine, işgücü gibi kaynaklarını;
· belirli miktarda
· istenilen kalitede,
· istenilen zamanda,
müşteri gereksinimlerini karşılayacak mal ve hizmetleri üretecek şekilde bir araya getirmesiyle ilgili işlemlerdir.Buradaki özellikler ihale kapsamında değerlendirlemez. BAB projeye veya özel istege göre ürün(mikroskop) üretecek donanım ve teknik bilgiye sahiptir
Üretim yönetimin rolü, firmamızın girdilerini tamamlanmış mal ve hizmetlere dönüştürmektir.
Girdileri ; insan kaynakları (çalışanlar, yöneticiler gibi), tesisler ve süreçler (bina ve ekipman gibi), malzeme - materyal, teknoloji ve bilgi oluşturur.
Çıktılar, firmamızın ürettiği mal ve hizmetlerdir.
Üretimdeki Bakış Açımız
Performans tasarımı, üstün özellikler, uzun ömür, sağlamlık, mükemmel müşteri hizmeti
Tasarım özelliklerini karşılamak, dar toleranslar, kusursuz teslim
Ürün tasarım kalitesi (mal ve hizmetin ihtiyaçları karşılaması)
Süreç kalitesi (tasarlandığı gibi üretilen hatasız ürünler)
Ürün esnekliği:
Üretimi hızla bir üründen başka bir ürüne değiştirebilme
Ürünü hızla müşteri isteklerine uydurabilme | Comparison of collagen biomatrix and omentum effectiveness on peripheral nerve regeneratio | Comparison of collagen biomatrix and omentum.....
Berker Cemil & Durukan Ture & Banu Cevirgen & Figen Kaymaz & Memduh Kaymaz
Department of Neurosurgery,
Gazi University School of Medicine,
Beşevler,
06500 Ankara, Turkey
Received: 22 January 2008 / Revised: 9 December 2008 / Accepted: 18 January 2009 # Springer-Verlag 2009
| IMMUNOHISTOCHEMICAL DISTRIBUTION OF ALPHA B-CRYSTALLIN IN THE CEREBELLUM OF DOGS INFECTED WITH CANINE DISTEMPER VIRUS | IMMUNOHISTOCHEMICAL DISTRIBUTION OF...
Tolga GUVENC*, Murat YARIM, Mustafa Yavuz GULBAHAR and Yonca B. KABAK
Department of Pathology, Faculty of Veterinary Medicine, University of Ondokuz Mayis,
Kurupelit, 55139 Samsun, Turkey
(Received 2 April 2007; accepted 6 September 2007)
| Yayın: Solution Cross-Linked Poly(isobutylene) Gels: Synthesis and Swelling Behavior | Yayın: Solution Cross-Linked Poly(isobutylene) Gels: Synthesis and Swelling Behavior
Oguz Okay,, Selda Durmaz, and Burak Erman*,§
TUBITAK Marmara Research Center, Department of Chemistry, P.O. Box 21, 41420 Gebze,
Kocaeli, Turkey, Istanbul Technical University, Department of Chemistry, 80626 Maslak, Istanbul,
Turkey, and Sabanci University, Faculty of Engineering and Natural Sciences, Orhanli, 81474 Tuzla,
Istanbul, Turkey
Received February 22, 2000; Revised Manuscript Received April 24, 2000
| Yayın: Solution Cross-Linked Poly(isobutylene) Gels: Synthesis and Swelling Behavior | Yayın: Solution Cross-Linked Poly(isobutylene) Gels: Synthesis and Swelling Behavior
Oguz Okay,, Selda Durmaz, and Burak Erman*,§
TUBITAK Marmara Research Center, Department of Chemistry, P.O. Box 21, 41420 Gebze,
Kocaeli, Turkey, Istanbul Technical University, Department of Chemistry, 80626 Maslak, Istanbul,
Turkey, and Sabanci University, Faculty of Engineering and Natural Sciences, Orhanli, 81474 Tuzla,
Istanbul, Turkey
Received February 22, 2000; Revised Manuscript Received April 24, 2000 | Yayın: Neuroprotective Effect of Erythropoietin on Hypoxic-Ischemic Brain Injury in Neonatal Rats | Yayın: Neuroprotective Effect of Erythropoietin on Hypoxic-Ischemic Brain Injury in Neonatal Rats
Abdullah Kumral a Erdener Ozer b Osman Yilmaz c Mustafa Akhisaroglu d
Necati Gokmen e Nuray Duman a Cagnur Ulukus b Sermin Genc f
Hasan Ozkana
Departments of aPediatrics, bPathology, cExperimental Animal Laboratory, dPhysiology,
eAnesthesiology and Reanimation, and fMedical Biology and Genetics, School of Medicine, Dokuz Eylul University,
Izmir, Turkey
| Yayın: Cyclosporine A Toxicity in Association With Reduced Renal Mass | Yayın: Cyclosporine A Toxicity in Association With Reduced Renal Mass
M. Gü ray, S. Sarıoğlu, M. Türkmen, O. Yılmaz, H. Ellidokuz, A. Gelal, H. Işlekel, A. Çoker, and E. Özer
| Yayın: Positive End-expiratory Pressure Reduces Pneumocephalus in Spinal Intradural Tumor Surgery | Yayın: Positive End-expiratory Pressure Reduces Pneumocephalus in Spinal Intradural Tumor Surgery
Journal of Neurosurgical Anesthesiology. 19(3):161-165, July 2007.
Turgut, Namigar MD *; Turkmen, Aygen MD *; Gokkaya, Sumru MD *; Hatiboglu, Mustafa Aziz MD +; Iplikcioglu, Ahmet Celal MD +; Altan, Aysel MD *
Abstract:
We tested the hypothesis that 5 cm H2O of positive end-expiratory pressure (PEEP) reduces the incidence of pneumocephalus in patients who undergo spinal intradural tumor surgery. Fifty-three ASA I to III patients who underwent thoracolumbar intradural tumor surgery between the years 2003 and 2006 were included in this study. All patients received propofol, fentanyl, and cisatracurium for induction of the anesthesia. Maintenance was provided by propofol infusion and, oxygen (50%) and air (50%). Group I (n=28) did not receive PEEP whereas group II (n=25) received PEEP as 5 cm H2O. Cranial computerized tomography was taken at 8 hours after the surgery and cases were evaluated for pneumocephalus using BAB Bs200ProP Image System software. Pneumocephalus areas between 0.03 and 4.24 cm2 were observed in 9 patients, 8 in group I and 1 patient in group II at the 8th postoperative hour, at various localizations. There were no neurologic findings in other patients except for 2 patients in group I who presented with headache and mental status change. Although the cerebrospinal fluid leakage is minimal, N2O is not used and the patients are well hydrated, pneumocephalus with neurologic deficits may occur in patients undergoing microsurgical spinal intradural tumor surgery in prone position. In our study, we showed that using 5 cm H2O PEEP perioperatively reduced the risk of pneumocephalus. However, more cases must be studied to support this hypothesis.
(C) 2007 Lippincott Williams & Wilkins, Inc. | Yayın: Interaction with intraocular lens materials: Does heavy silicone oil act like silicone oil? | Yayın: Interaction with intraocular lens materials: Does heavy silicone oil act like silicone oil?
Aylin Yaman, MD, A. Osman Saatci, MD, Sulen Sarıog?lu, MD, F. Hakan O¨ ner, MD, Ismet Durak, MD
PURPOSE: To determine the interaction of heavy silicone oil with various intraocular lens (IOL) materials and whether heavy silicone oil covers the silicone IOL optic as silicone oil does. SETTING: Department of Ophthalmology, Dokuz Eylul University, Izmir, Turkey. METHODS: The study group comprised 5 poly(methyl methacrylate) (PMMA) IOLs, 4 foldable silicone IOLs, 5 foldable hydrophilic acrylic IOLs, and 5 foldable hydrophobic acrylic IOLs. Each IOL was bathed in balanced salt solution (BSS) for 10 minutes and then placed in heavy silicone oil dyed with Sudan Black for another 10 minutes. Afterward, each IOL was reimmersed in BSS for 5 minutes and examined under the light microscope. Digital images were analyzed to determine the optic area covered with heavy silicone oil. | Yayın : Effects of lactational cyclosporine A use on rat pups | Pediatric Transplantation
Yayın : Effects of lactational cyclosporine A use on rat pups
Volume 10 Issue 4 Page 454-460, June 2006
To cite this article: Mehmet Atilla Türkmen, Salih Kavukçu, Sülen Sarıoglu, Alper Soylu, Hülya Akhunlar, Osman Yılmaz, Hülya Güven (2006)
Effects of lactational cyclosporine A use on rat pups
Pediatric Transplantation 10 (4), 454460.
doi:10.1111/j.1399-3046.2006.00495.x
Abstract
Effects of lactational cyclosporine A use on rat pups
Mehmet Atilla Türkmen11Dokuz Eylül University Medical Faculty, Division of Pediatric Nephrology, 35340, Inciralti, Izmir, Turkey, Salih Kavukçu11Dokuz Eylül University Medical Faculty, Division of Pediatric Nephrology, 35340, Inciralti, Izmir, Turkey, Sülen Sarıolu22Dokuz Eylül University Medical Faculty, Department of Pathology, 35340, Inciralti, Izmir, Turkey, Alper Soylu11Dokuz Eylül University Medical Faculty, Division of Pediatric Nephrology, 35340, Inciralti, Izmir, Turkey, Hülya Akhunlar33Dokuz Eylül University Medical Faculty, Department of Biochemistry, Turkey, Osman Yılmaz44Dokuz Eylül University Medical Faculty, Animal Research Center, Izmir, Turkey and Hülya Güven55Dokuz Eylül University Medical Faculty, Department of Pharmocology, Izmir, Turkey1Dokuz Eylül University Medical Faculty, Division of Pediatric Nephrology, 35340, Inciralti, Izmir, Turkey, 2Dokuz Eylül University Medical Faculty, Department of Pathology, 35340, Inciralti, Izmir, Turkey, 3Dokuz Eylül University Medical Faculty, Department of Biochemistry, Turkey, 4Dokuz Eylül University Medical Faculty, Animal Research Center, Izmir, Turkey, 5Dokuz Eylül University Medical Faculty, Department of Pharmocology, Izmir, Turkey
Abstract
Abstract: We aimed to evaluate the structural and functional changes in the thymus and kidneys of rat pups whose mothers were given cyclosporine A (CsA) during lactational period. Six adult nursing Wistar rats and their 30 pups were studied. Rat pups were divided into four groups as follows: 21-day treated group and 21-day placebo group, each including 10 breastfeeding pups sacrificed on the 21st day, whose mothers were given CsA or placebo, respectively (infancy groups) and, 60-day treated group and 60-day placebo group, each including five breastfeeding pups sacrificed on the 60th day, whose mothers were given CsA or placebo, respectively (puberty groups). While CsA levels of mother rats were very high, CsA levels of 21-day treated group pups were zero. There were no renal histomorphometric differences between study and control pups in both age groups. Renal function parameters showed significant differences between study and control pups in the infancy group: the 21-day treated group pups had significantly lower urine volume, proteinuria, FENa and urinary NAG/creatinine ratio. GFR was also lower in the 21-day treated group, but the difference was not significant, and serum creatinine levels were also not different. Renal function differences were not present among the pubertal pups. Thymic corticomedullary ratio of the 21-day treated group was significantly higher than the 21-day placebo group, while there was no difference between the 60-day treated group and 60-day placebo group. There were no significant changes in the number and distribution of CD3+, CD4+, and CD8+ thymocytes between study and control pups in both age groups. In conclusion, breastfeeding by CsA-treated mother rats induced structural alterations in the thymus and functional changes in the kidneys of the rat pups during infancy. Disturbances in the kidneys and thymus mostly improved after CsA exposure was over | Interaction of Intraocular Lenses with... |  | Lymphocyte DNA damage in Turkish
asphalt workers detected by the comet
Lymphocyte DNA damage in Turkish
asphalt workers detected by the comet
Lymphocyte DNA damage in Turkish
asphalt workers detected by the comet
Lymphocyte DNA damage in Turkis | Paper: Lymphocyte DNA damage in Turkish asphalt workers detected by the comet assay
International Journal of Environmental Health Research
Publication details, including instructions for authors and subscription information:
http://www.tandfonline.com/loi/cije20
Lymphocyte DNA damage in Turkish asphalt workers detected by the comet assay
Aysegul Bacaksiz a , Zeliha Kayaalti b , Esma Soylemez b , Engin
Tutkun c & Tulin Soylemezoglu b
a Department of Nutrition and Dietetics , Fazil Boyner School of
Health, Kastamonu University , Kastamonu , Turkey
b Institute of Forensic Sciences, Ankara University , Ankara ,
Turkey
c Ankara Occupational Diseases Hospital , Ankara , Turkey
Published online: 03 May 2013. | Effect of cigarette smoking on DNA damage according to nine comet assay parameters in female and male groups | Paper: Dokuz comet parametresine göre kadın ve erkek gruplarda sigara içiminin DNA hasarına etkisi
Dokuz comet parametresine göre kadın ve erkek gruplarda sigara içiminin DNA hasarına etkisi
Esma Söylemez, Zeliha Kayaaltı, Vugar Aliyev, Tülin Söylemezoğlu
Ankara Üniversitesi Adli Bilimler Enstitüsü
*This study is presented as poster in 47th Congress of the European
Societies of Toxicology, 2011, Paris, France.
Aim: Smoking poses a serious threat to public health. The aim of this study was to investigate
the relationship between the smoking and DNA damage in lymphocytes. A potential genotoxic
effect of cigarette smoking was analyzed with the nine comet assay parameters including
comet length (CL), comet intensity (CI), head length (HL), head intensity (HI), tail length (TL), tail
intensity (TI), DNA tail (DNAt), tail moment (TM) and olive tail moment (OTM). For the first time
in this study, | Bs Ortopedi | Yayın:displazili köpeklerin Norberg-Olsson ve distraksiyon indeksi kalça eklem açılarının ölçülmesi
Vet Hekim er erg 82(1): 49-58, 2011 Araştırma Makalesi / esearch Article Bilgisayar programı ile kalça displazili köpeklerin Norberg-Olsson ve distraksiyon indeksi kalça eklem açılarının ölçülmesi ve alınan sonuçların değerlendirilmesi Hasan SAI*, Hasan BİGİİ** Öz: Bu çalışmada, 100 adet kalça eklem radyografisinde Norberg-Olsson Açısı ve istraksiyon İndeksi değerlerinin ölçümü yeni bir bilgisayar programı (Bs200Pro Software-BsCelik, BAB igital Imaging System 2007, Ankara, Türkiye) ile yapıldı. Olguların radyografileri ventro/dorsal ve distraksiyon pozisyonunda genel anestezi altında alındı. Norberg-Olsson açılarının değerlendirilmesinde Bilgili-Sarı eğerlendirme Skalası kullanıldı. istraksiyon İndeksi değeri arasında olanlar negatif, arasında olanlar şüpheli, 0.5 in üzerinde olanlar ise pozitif olarak değerlendirildi. Bu değerlendirme sonrasında 100 kalça radyografisinden %69 unda displazi pozitif izlenirken, %31 inde displazi negatif olarak izlendi. Bu uyumsuzluklar oranı distraksiyon radyografileri ile karşılaştırıldığında 100 kalça radyografisinden %50 sinde displazi pozitif belirlenirken, %27 sinde şüpheli, %23 ünde de negatif olarak belirlendi. | Determination of the effects of some artificial sweeteners on human peripheral Lymphocytes using the comet assay |
Yayın :Determination of the effects of some artificial sweeteners on human peripheral lymphocytes using the comet assay
Zeynep Fındıklı and ifa Türkoğlu
Department of Biology, Faculty of Arts and Science, Cumhuriyet University, Sivas, Turkey.
Received 02 August 2014; Accepted 11 September 2014
In this study, the genotoxicity of the artificial sweeteners acesulfame potassium, aspartame, saccharin,
and sorbitol, which are used in food industry and by patients with diabetes, was investigated in human
peripheral lymphocyte cells using the single-cell gel electrophoresis (comet) technique. Human
lymphocyte cells were treated with the substances for three hour at each of the three dosages (1.25, 2.5,
and 5 ppm). The chemical additives were studied, and the related DNA damages in the study group were
compared to the control group for each of the treatment dosages. The DNA breakages observed in the
comet assay were assessed in terms of tail moment and tail DNA percent using the comet parameters.
The statistical and photographic analyses were performed using SPSS 15 and BAB BS 200 Pro
software, respectively. Based on the results for the short-term in vitro treatments, the 4 different food
flavorings were found to have genotoxic effects.
ACKNOWLEDGEMENTS
This study was supported by the Cumhuriyet University
Coordination Unit of Scientific Research Projects (Project
Code: F-325). The authors are grateful to Dr. Serpil Ünver Saraydõn (for the fluorescence microscope) and
Babacan Uğuz (for the BAB Bs200ProP/BsComet DNA Comet Assay).
| Ki67 expression in the cerebellum of dogs with distemper | Paper:Ki67 expression in the cerebellum of dogs with distemper
Dokuz comet parametresine göre kadın ve erkek gruplarda s
Department of Pathology, Faculty of Veterinary Medicine, University of Ondokuz Mayis, 55139 Samsun, TURKEY.
*Corresponding author: myarim@omu.edu.tr | Türkiye’de Doğal Olarak Yetişen Amelanchier Medik. Taksonlarının Odun Anatomileri | Paper: Türkiye’de Doğal Olarak Yetişen Amelanchier Medik. Taksonlarının Odun Anatomileri
Bedri SERDAR* Mustafa KARAKÖSE Reha MAZLUM
Murat ÖZTÜRK Çağdaş GÖL Salih TERZİOĞLU
Karadeniz Teknik Üniversitesi Orman Fakültesi Orman Mühendisliği Bölümü, Trabzon
*Sorumlu Yazar: Geliş Tarihi: Ağustos 11, 2014 | Endometrial echotexture parameters in Turkish Saanen Goats
(Akkeci) during oestrus and early pregnancy |
Paper: Animal Reproduction Science
Endometrial echotexture parameters in Turkish Saanen Goats (Akkeci) during oestrus and early pregnancy
M. Cengiza,∗, H. Kancab, S. Salarb, A. Bastanb, I. Kucukaslanc, H. Alkanb,
K. Karakasb, O. Yukselb, A. Hayirli d
a Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, Atatürk University, 25240 Erzurum, Turkey
b Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, Ankara University, 06110 Ankara, Turkey
c Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, Dicle University, 21300 Diyarbakir, Turkey
d Department of Animal Nutrition and Nutritional Disorders, Faculty of Veterinary Medicine, Atatürk University, 25240 Erzurum, Turkey
| Endometrial echotexturevariablesinpostpartumcowswith subclinical endometritis |
Paper: Endometrial echotexture variables in postpartum cows with subclinical endometritis subclinical endometritis
Endometrial echotexturevariablesinpostpartumcowswith subclinical endometritis
B. Polata,∗, M.Cengiza, O.Cannazika, A.Colaka, E.Orucb, S.Altunb, S.Salarc,
A. Bastanc
a Department ofObstetricsandGynecology,FacultyofVeterinaryMedicine,AtatürkUniversity,25240Erzurum,Turkey
b Department ofPathology,FacultyofVeterinaryMedicine,AtatürkUniversity,25240Erzurum,Turkey
c Department ofObstetricsandGynecology,FacultyofVeterinaryMedicine,AnkaraUniversity,06110Ankara,Turkey
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