Yayın : Detection of irradiated quail meat by using DNA comet assay and evaluation of comets by image analysis

by: Yakup Erel, Nizamettin Yazici, Sumer Özvatan, Demet Ercin, Nurcan Cetinkaya
Radiation Physics and Chemistry (02 June 2009)

Abstract

A simple technique of microgel electrophoresis of single cells (DNA Comet Assay) was used to detect DNA comets in irradiated quail meat samples. Obtained DNA comets were evaluated by both photomicrographic and image analysis.Quail meat samples were exposed to radiation doses of 0.52, 1.05, 1.45, 2.00, 2.92 and 4.00 kGy in gamma cell (Gammacell 60 Co, dose rate 1.31 kGy/h) covering the permissible limits for enzymatic decay and stored at 2 o C. The cells isolated from muscle (chest, thorax) in cold PBS were analysed using the DNA comet assay on 1, 2, 3, 4, 7, 8 and 11 day post irradiation. The cells were lysed between 2, 5 and 9 min in 2.5% SDS and electrophroesis was carried out at a voltage of 2 V/cm for 2 min. After propidium iodide staining, the slides were evaluated through a fluorescent microscope. In all irradiated samples, fragmented DNA stretched towards the anode and damaged cells appeared as a comet. All measurement data were analyzed using BS 200 ProP with software image analysis (BS 200 ProP, BAB Imaging System, Ankara, Turkey). The density of DNA in the tails increased with increasing radiation dose. However, in non-irradiated samples, the large molecules of DNA remained relatively intact and there was only minor or no migration of DNA; the cells were round or had very short tails only. The values of tail DNA %, tail length and tail moment were significantly different and identical between 0.9 and 4.0 kGy dose exposure, and also among storage times on day 1, 4 and 8. In conclusion, DNA Comet Assay EN 13784 standard method may be used for not only screening method for detection of irradiated quail meat depending on storage time and condition but also for the quantification of applied dose if it is combined with image analysis.Image analysis may provide a powerful tool for the evaluation of head and tail of comet intensity related with applied doses.

Passive smoking



BAB Bs200 Pro Comet Image Process and Analysis Software used on this microscope to calcute

PERFORMANCE EVALUATION OF DRUG APPLICATION ...


Bahadır SAYINCI* Saim BASTABAN
Faculty of Agriculture, 25240 Erzurum Ataturk University Department of Agricultural Machinery

*e-mail: bsayinci@atauni.edu.tr

Geliş Tarihi: 20.10.2008 Kabul Tarihi: 10.03.2009


INVESTIGATION OF QUALITATIVE AND QUANTITATIVE ANALYSIS METHODS IN EVALUATING
SPRAY APPLICATION PERFORMANCE

ABSTRACT: Various qualitative and quantitative methods were developed for the determination of spray application performance. These methods are desired to be practical, easy and economic because of analyzing lots of samples to decrease error variance. The measurements in quantitative methods were made with image processing software. Water-sensitive, oil-sensitive and kromekote cards are used as sampling materials and their image processing quality is spoiled by changing color at the unsuitable conditions because of their sensitivity to humidity. Sampling materials such a filter paper, petri dishes and plant leaf are used in quantitative methods and food dye is mixed to spray liquid instead of pesticide in the spray application. At this method, the mixing of dye completely washed from surface without degradation, depends on the properties of the used sampling material. On the other hand, dye deposited on the surface after the application may be degraded due to its explosion to solar radiation at the open atmosphere and this can change the concentration of solution. In this study, the factors affecting the recovery of color dyes, degradation of dye materials and image processing quality in qualitative and quantitative methods were reviewed regarding up to date literatures.
Keywords: Image Processing Analysis, Water Sensitive Paper, Degradation of Dye, Spray Recovery

Comparison of collagen biomatrix and omentum.....

Berker Cemil & Durukan Ture & Banu Cevirgen & Figen Kaymaz & Memduh Kaymaz

Department of Neurosurgery,

Gazi University School of Medicine,

Beşevler,

06500 Ankara, Turkey

Received: 22 January 2008 / Revised: 9 December 2008 / Accepted: 18 January 2009 # Springer-Verlag 2009

IMMUNOHISTOCHEMICAL DISTRIBUTION OF...


Tolga GUVENC*, Murat YARIM, Mustafa Yavuz GULBAHAR and Yonca B. KABAK

Department of Pathology, Faculty of Veterinary Medicine, University of Ondokuz Mayis,

Kurupelit, 55139 Samsun, Turkey

(Received 2 April 2007; accepted 6 September 2007)

Yayın: Solution Cross-Linked Poly(isobutylene) Gels: Synthesis and Swelling Behavior

Oguz Okay,†,‡ Selda Durmaz,† and Burak Erman*,§
TUBITAK Marmara Research Center, Department of Chemistry, P.O. Box 21, 41420 Gebze,
Kocaeli, Turkey, Istanbul Technical University, Department of Chemistry, 80626 Maslak, Istanbul,
Turkey, and Sabanci University, Faculty of Engineering and Natural Sciences, Orhanli, 81474 Tuzla,
Istanbul, Turkey
Received February 22, 2000; Revised Manuscript Received April 24, 2000

Yayın: Solution Cross-Linked Poly(isobutylene) Gels: Synthesis and Swelling Behavior

Oguz Okay,†,‡ Selda Durmaz,† and Burak Erman*,§
TUBITAK Marmara Research Center, Department of Chemistry, P.O. Box 21, 41420 Gebze,
Kocaeli, Turkey, Istanbul Technical University, Department of Chemistry, 80626 Maslak, Istanbul,
Turkey, and Sabanci University, Faculty of Engineering and Natural Sciences, Orhanli, 81474 Tuzla,
Istanbul, Turkey
Received February 22, 2000; Revised Manuscript Received April 24, 2000

Yayın: Neuroprotective Effect of Erythropoietin on Hypoxic-Ischemic Brain Injury in Neonatal Rats

Abdullah Kumral a Erdener Ozer b Osman Yilmaz c Mustafa Akhisaroglu d
Necati Gokmen e Nuray Duman a Cagnur Ulukus b Sermin Genc f
Hasan Ozkana
Departments of aPediatrics, bPathology, cExperimental Animal Laboratory, dPhysiology,
eAnesthesiology and Reanimation, and fMedical Biology and Genetics, School of Medicine, Dokuz Eylul University,
Izmir, Turkey

Yayın: Cyclosporine A Toxicity in Association With Reduced Renal Mass

M. Gü ray, S. Sarıoğlu, M. Türkmen, O. Yılmaz, H. Ellidokuz, A. Gelal, H. Işlekel, A. Çoker, and E. Özer

Yayın: Positive End-expiratory Pressure Reduces Pneumocephalus in Spinal Intradural Tumor Surgery

Journal of Neurosurgical Anesthesiology. 19(3):161-165, July 2007.
Turgut, Namigar MD *; Turkmen, Aygen MD *; Gokkaya, Sumru MD *; Hatiboglu, Mustafa Aziz MD +; Iplikcioglu, Ahmet Celal MD +; Altan, Aysel MD *

Abstract:
We tested the hypothesis that 5 cm H2O of positive end-expiratory pressure (PEEP) reduces the incidence of pneumocephalus in patients who undergo spinal intradural tumor surgery. Fifty-three ASA I to III patients who underwent thoracolumbar intradural tumor surgery between the years 2003 and 2006 were included in this study. All patients received propofol, fentanyl, and cisatracurium for induction of the anesthesia. Maintenance was provided by propofol infusion and, oxygen (50%) and air (50%). Group I (n=28) did not receive PEEP whereas group II (n=25) received PEEP as 5 cm H2O. Cranial computerized tomography was taken at 8 hours after the surgery and cases were evaluated for pneumocephalus using BAB Bs200ProP Image System software. Pneumocephalus areas between 0.03 and 4.24 cm2 were observed in 9 patients, 8 in group I and 1 patient in group II at the 8th postoperative hour, at various localizations. There were no neurologic findings in other patients except for 2 patients in group I who presented with headache and mental status change. Although the cerebrospinal fluid leakage is minimal, N2O is not used and the patients are well hydrated, pneumocephalus with neurologic deficits may occur in patients undergoing microsurgical spinal intradural tumor surgery in prone position. In our study, we showed that using 5 cm H2O PEEP perioperatively reduced the risk of pneumocephalus. However, more cases must be studied to support this hypothesis.

(C) 2007 Lippincott Williams & Wilkins, Inc.

Yayın: Interaction with intraocular lens materials: Does heavy silicone oil act like silicone oil?



Aylin Yaman, MD, A. Osman Saatci, MD, Sulen Sarıog?lu, MD, F. Hakan O¨ ner, MD, Ismet Durak, MD



PURPOSE: To determine the interaction of heavy silicone oil with various intraocular lens (IOL) materials and whether heavy silicone oil covers the silicone IOL optic as silicone oil does. SETTING: Department of Ophthalmology, Dokuz Eylul University, Izmir, Turkey. METHODS: The study group comprised 5 poly(methyl methacrylate) (PMMA) IOLs, 4 foldable silicone IOLs, 5 foldable hydrophilic acrylic IOLs, and 5 foldable hydrophobic acrylic IOLs. Each IOL was bathed in balanced salt solution (BSS) for 10 minutes and then placed in heavy silicone oil dyed with Sudan Black for another 10 minutes. Afterward, each IOL was reimmersed in BSS for 5 minutes and examined under the light microscope. Digital images were analyzed to determine the optic area covered with heavy silicone oil.

Pediatric Transplantation

Yayın : Effects of lactational cyclosporine A use on rat pups



Volume 10 Issue 4 Page 454-460, June 2006

To cite this article: Mehmet Atilla Türkmen, Salih Kavukçu, Sülen Sarıoglu, Alper Soylu, Hülya Akhunlar, Osman Yılmaz, Hülya Güven (2006)
Effects of lactational cyclosporine A use on rat pups
Pediatric Transplantation 10 (4), 454–460.
doi:10.1111/j.1399-3046.2006.00495.x
Abstract
Effects of lactational cyclosporine A use on rat pups
Mehmet Atilla Türkmen11Dokuz Eylül University Medical Faculty, Division of Pediatric Nephrology, 35340, Inciralti, Izmir, Turkey, Salih Kavukçu11Dokuz Eylül University Medical Faculty, Division of Pediatric Nephrology, 35340, Inciralti, Izmir, Turkey, Sülen Sarıolu22Dokuz Eylül University Medical Faculty, Department of Pathology, 35340, Inciralti, Izmir, Turkey, Alper Soylu11Dokuz Eylül University Medical Faculty, Division of Pediatric Nephrology, 35340, Inciralti, Izmir, Turkey, Hülya Akhunlar33Dokuz Eylül University Medical Faculty, Department of Biochemistry, Turkey, Osman Yılmaz44Dokuz Eylül University Medical Faculty, Animal Research Center, Izmir, Turkey and Hülya Güven55Dokuz Eylül University Medical Faculty, Department of Pharmocology, Izmir, Turkey1Dokuz Eylül University Medical Faculty, Division of Pediatric Nephrology, 35340, Inciralti, Izmir, Turkey, 2Dokuz Eylül University Medical Faculty, Department of Pathology, 35340, Inciralti, Izmir, Turkey, 3Dokuz Eylül University Medical Faculty, Department of Biochemistry, Turkey, 4Dokuz Eylül University Medical Faculty, Animal Research Center, Izmir, Turkey, 5Dokuz Eylül University Medical Faculty, Department of Pharmocology, Izmir, Turkey

Abstract
Abstract: We aimed to evaluate the structural and functional changes in the thymus and kidneys of rat pups whose mothers were given cyclosporine A (CsA) during lactational period. Six adult nursing Wistar rats and their 30 pups were studied. Rat pups were divided into four groups as follows: 21-day treated group and 21-day placebo group, each including 10 breastfeeding pups sacrificed on the 21st day, whose mothers were given CsA or placebo, respectively (infancy groups) and, 60-day treated group and 60-day placebo group, each including five breastfeeding pups sacrificed on the 60th day, whose mothers were given CsA or placebo, respectively (puberty groups). While CsA levels of mother rats were very high, CsA levels of 21-day treated group pups were zero. There were no renal histomorphometric differences between study and control pups in both age groups. Renal function parameters showed significant differences between study and control pups in the infancy group: the 21-day treated group pups had significantly lower urine volume, proteinuria, FENa and urinary NAG/creatinine ratio. GFR was also lower in the 21-day treated group, but the difference was not significant, and serum creatinine levels were also not different. Renal function differences were not present among the pubertal pups. Thymic corticomedullary ratio of the 21-day treated group was significantly higher than the 21-day placebo group, while there was no difference between the 60-day treated group and 60-day placebo group. There were no significant changes in the number and distribution of CD3+, CD4+, and CD8+ thymocytes between study and control pups in both age groups. In conclusion, breastfeeding by CsA-treated mother rats induced structural alterations in the thymus and functional changes in the kidneys of the rat pups during infancy. Disturbances in the kidneys and thymus mostly improved after CsA exposure was over